Abstract

In recent years, carbapenem-resistant Enterobacterales (CRE) has emerged as a more abundant and difficult-to-treat pathogen. Colistin seemed to be the  last-resort antibiotic.We treated an eighty-year-old male who had colistin-resistant and carbapenem resistant Klebsiella pneumoniae bacteremia during hospitalization. Since there was no effective antibiotic available, the patient expired in 2019. We later used next-generation sequencing (NGS) on the patient's blood isolate to identify the resistant mechanisms of the pathogen. The bla_OXA-48 resistant gene could indicate resistance to carbapenem. NGS technology became available in our research laboratory in 2024 to precisely detect antimicrobial resistance. In comparison with rapid BioFire FilmArray system, NGS could be used to precisely detect antimicrobial resistance and could be considered as one of the diagnostic tools to improve patient care.